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No hypochlonte has to be added to the receiver since the chlonde content of the sample and of the fuimel washings furnish a large excess of hypochlonte.

The ondation is performed diroctly in the rccen er after the ground gloss part and the glase tubing have been nnaed carefuDj One gnun of bone acid and 0,5 Gm.

The tube la iihftLfm wnd 0. CaleulaUon A blank dctennmatlon la made whenever new reagents are pre- liarcd, the blank ordinarily ranges between 1 0 and 1 7 ml of thlcK sulfate and should bo subtracted from the volume of thiosulfate used m the analysis.

The organic substance is destroyed with chromic acid sulfunc acid m the presence of ccnc sulfate as a cata IjTjt.

The flask is allowed to cool, first by itself, than by outeido coolmg, it IS then carefully diluted mth water, boDed ogam to rmove chromyl chloride, and again diluted with water to prevent tho dmclopment of SOj vnporB during distillation Now tho flask is connected to the apparatus.

Now the ansemoufl acid is added through the dropping funnel at the rate of drops per second. However these amounts will not normally occur m blood When small amounts of iodine, such as normally found m blood, are to be determmed the reduemg agent Is added until the contents of the flask turn blue-green.

This point can easily be recognised oven by an untramed observer An excess of arsemoua odd la not harmful because the lodme is libemtod instantaneously from the diction mixture.

Prolonged distillation is therefore unnecessary Clomp Hi Is now opened as far os the pressure pennits and the condenser tube becomes gradually hot all the way to the bottom of the rocciver In this way any traces of lodme left m the condenser are vaponied and can combmo with the NaOH in the receiver v ess e l s Clomp Hi IS closed m order to allow nnsing of the condcaiser tube with the receiver fluid and It is opened again to allow the fluid to return to the flask.

Ihe condenser tube is finally washed out by the condensing water vapors. Ftnallv after the elution stood for 2 mu drop of urea C to the 1 ml.

Froa 0 mL of potasaum iodide solution b then added to the taming 'up to 1 mg. After addmi tie permanganate, rotate the solution gently upon the sides d the flask and heat tho saraples for two romutes Then add 2 dn?

The iodme is liberated from the lodato by tho addition of iodide and titrated with sodrum thiosulfate. The contents must be clear and colorless, otheiTiiae a few more drops of sulfunc acid must be added The hot flask is put back on the flame and dDuto KNlnOi solution 3 IS added till the pink color remains.

S mg of phosphorus are added to 80 ml of 10 per cent tnchloracetio acid solution m a mh volumetno [lask. The solution keeps for approximately 2 weeks, when kept cold and in the dark icebox pToadurt A test tube with ground glass top is filial with 8 ml of tnchlor acetic acid and 2 mL of oxalaCed blood is added with ahaking After continued shakmg it b filtered through an ash-free filter Five mL of filtrate correspondmg to 1 ml of blood 1 mL of molybdate and 0 4 mL of ammosulfomo add reagent are pipetted into a 10 mL vdu mstno flfiak, water added up to the mark and the contenta weD muted.

At the same tune 6 ml of the working standard phosphate solutioa a or is pipetted into a 10 mL volumetne OMk, 1 mJ of molybdate and 0 4 ml of ammoaulfoDio acid reagent are added, and made up with water to the mark.

P y w colorimeter reading. The rate of dilution can be found by dotermiaing the concentratjon of the stock solution as follows mto a 60 mL measuring flask is placed 0 1 ml of SdCIj and made up to the mark with distilled water Test tubes of 2J5 cm.

With stining 0. Further detenmnatioa and calculatMn is earned out according to directions for the deter mmatlon of phosphorus m serum.

Filter if cloudy For all other reagents see determination of inorganic phosphorus. If the digestlonismcomplete a black residue remainfl In this case 0J2 ml of fresh HtOt must be added and the digestion continued.

Unknown and standard are now dOutod to the 8 ml mark with redistilled water and 1 ml of molybdate reagent 3 and 1 mb of dilute SnDi solution is added.

If tho Hdhge colorimeter is used the method is the same as de- scribed above Solution 0 serves as standard.

For all other reagents see determination of total acid-soluble phosphorus Proaditn Into a 10 ml volumetno flask ore placed approximately 0 ml of the alcohol-ether mixture.

Slowly and with shaking 0. Values of 8 mg pier cent and above can be found m this disease Similar changes occur m nephrosclerosis, in cystic kidneys, in pyonephrosis, hjilronephrosis, ond m tuberculosis of the kidney 4 During healing of bone fractures the phosphate levd is in creased.

An mcrcoso m phosphorus is a sign of kidney msuflicicncy and is often found together with a decrease of alkali- reservT Values of 8 mg per coot end above can be found in this disease Similar changes occur in nephrosclerosis, m ej'stk kMnoys, m pjoncphroeifl, hvdroncpbrosis and m tuberculosis of the kidney 4 Durmg healing of bone fractures tho phosphate level is lu creased.

Durmg this penod values may vary between 6 and 7 mg per cent Low scrum levels are found m I Rickets In children values between 1 and 2 mg pcf have been encountered.

ExampU 0. The air should bo sucked through the mixture kept in a narrow tube. Both solutions can be kept on ice for a long time.

Therefore it IB difBcult to give exact dirootions for the preparation of a standard solution. This procedure is repeated tonos until the supernatant is colorless.

After removal of the water as described above, 6 ml of ceno reagent 2 IS added and the tubes ore heated m a boiling water bath for approximately tuo minutes to dissolve the precipitate Thin glass rods ore used for stimng If n suitable metal rack for holding the tubes Is available, all samples may bo boiled at the same time.

After coolmg the contents of the centrifuge tubes are transferred quan titatively mto Hagedom-Jenaen tubes. Whea large centrifuge tubes are used fig 24 rthe titrarion-maybe perfenned directly m the centrifuge tubes.

E-xact potassium values can only be obtained when tho scrum is completely free of hemoglobin. Principle of the mdhod Calcium is precipitated as calcium oxalate in serum or plasma.

Example ThlcMuliate used up by the blank 1 09 ml thloaolfate used up by the aample 0. In order to prevent any precipita tion of magnesium when ammomum oxalate is added, drops of acetic acid are added until the green color changes back to red.

Three ml of ammonia solu tion arc added the tubes ogam centnfuged and the supernatant removed as described above. This procedure is repeated tmee more The precipitate must not be stirred up durmg tho process of washmg Now the precipitate is dissolved m 0.

The phoephorua content of the precipitate la detenmned and from this value tho magnesium content of tlie ongmol aample a cal ciliated. ReagenU 1 cold saturated ammonium oxalate solution 2 2 per cent ammonium phosphate Bolution 3 concentrated ammonia MflJnly according to W Donnie Journ Biol Chem 51 One mb of 10 N HiSOi ts slowlj added to the hot sample to dissolve the preerpilate After cooling it a mode up to the mati.

Now the sample is filtered through a Pregl filter fig 25a while still hot To avoid the formation of a precipitate along the walls of the filter tubing the apparatus is nnsed uith hot water immediately be- fore use.

A high magne- sium lev cl can bo encountered in mvo'Uicnla and during pcnodic paml VMS of tho extremities which maj be connected with an occasiona!

To each flask 3 ml of sodium acetate and bromme-glacml acetic acid is added from a droppmg flask until the brown color bromme persists.

After each determination the apparatus must be washed with re- distilled ivater, the water being sucked through the inner tube of the condenser with a water pump If organs are to be digested 1 ml of concentrated nitnc acid is added to the digestion acid and after the water has evaporated the sample is treated as described above.

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